Assay Development Guide to Protein:Protein Studies
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چکیده
Interactions between proteins are a key feature of many biochemical processes, for example cell signalling. In the absence of any enzymatic activity, measurement of protein:protein interactions has presented problems. Scintillation Proximity Assay (SPA) technology permits the direct measurement of binding of one protein to another. In addition, in cases where the dissociation rate of the interaction is high, SPA provides a means of equilibrium counting in an homogeneous assay format. An SPA system for the measurement of the direct binding of the GTP-ase activating protein, Neurofibromin-1 (NF1) to an oncogenic Ras protein (Leu-61 Ras) has been developed(1). The assay development and optimization process is used to illustrate the key features in designing a protein-protein interaction SPA. SPA Technology a p p l i c a t i o n n o t e
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